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KMID : 0603520000050030126
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Journal of Korean Association of Cancer Prevention
2000 Volume.5 No. 3 p.126 ~ p.134
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Anti-clastogenicity Study of Ellagic Acid - In Vitro Chromosome Aberration and Micronucleus Assay in Chinese
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Oh Hye-Young
Kim Jong-Won
Park Chang-Hwan
Han Eui-Sik
Sohn Soo-Jung
Kang Il-Hyun
Kim In-Sook
Ha Kwang-Won
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Abstract
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Dietary anticarcinogens/mutagens have been demonstrated to significantly reduce the incidence of cancers in animals and humans. The ellagic acid (EA) is a naturally occurring plant phenol which has been shown to reduce the carcinogenicity and mutagenicity of a variety of carcinogens, including N-nitrosomethylurea, benzo[a]pyrene,
aflatoxin B1. The chromosome aberration assay using cultured mammalian cells is one of the most sensitive methods to predict environmental mutagens and/or carcinogens, and is a complementary test to the Samonella/microsome assay (Ames test), and the in vitro micronucleus assay is a mutagenicity test system for the detection of chemicals which induce the formation of small membrane bound DNA fragment i.e. micronuclei in the cytoplasm of interphase cells. The in vivo micronucleus assay, which examines metaphase chromosomes from the bone marrow cells of rodents exposed to a test chemical just before sacrifice, is a powerful tool and cytogenetic assay. This assay may be used to detect damage to actively dividing cells not only from experimental test substances, but also from their metabolites as they are formed within the test animal. In this study, EA was treated in chinese hamster lung fibroblast (CHL) cells using in vitro chromosome aberration assay, in vitro micronucleus assay, and in ddY mice using in vivo micronucleus assay for their inhibitory effects against mitomycin C (MMC)-induced genotoxicity. The frequencies of chromosome aberrations and micronuclei were found to be decreased following EA treatment. On the basis of our results, we found that the EA had inhibitory effects on MMC-induced genotoxicity and we concluded that these test systems are useful and sensitive for screening of antimutagens.
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KEYWORD
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Ellagic acid, Anti-clastogen, Anti-mutagen, Chromosome aberration assay, In Vitro micronucleus assay
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